The Sony SH800Z Sorting Flow Cytometer is a compact, easy to use instrument that performs both cell counting and two-way cell sorting. The integrated laser engine houses 4 lasers, providing excitation lines of 405 nm, 488 nm, 561 nm and 638 nm. These lasers are combined and delivered through a single fiber while 6 freeform PMTs allow for the detection of fluorescence signals from any laser based on filter selection.
Manufacturer: Sony
Model No.: SH-800Z
Year of manufacture: 2016
Year acquired: 2016
Location: Parsons Room W118
Specifications:
- Optics
- Excitation Lasers: 405nm, 488nm, 561nm, 638nm
- Output Power: 30mW (max.) optical fiber output
- Beam Alignment: Collinear optical system
- Detection Parameters: 6 Fluorescent + 2 Scatter
- Pulse Measurement: Height, area, width
- Fluidics
- Single, auto-loading tube
- Sample Tube Types: 0.5ml, 1.5ml, 5 ml, 15 ml tubes
- Sort Streams: 2
- Sort Collection Devices: 2-way tube (5ml, 15ml), multiwell plates, PCR tubes, slides
- Temperature Control: Pre-sort -- 5 °C, 37 °C, Post-sort -- 5 °C
- Nozzle Sizes: 70, 100, 130 µm
- Performance
- Event Rate: 100,000 events per second max
- Sorting Speed: Using the 70 µm sorting chip at 50kHz, an average threshold of 12,000 events per second can be achieved with >95% purity and >80% yield. The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson's statistics. Using 100 µm or 130 µm sorting chips, maximum sorting speed is 10,000 events per second.
- Index Sorting: Yes
- Scatter Resolution: 0.5 µm
- Fluorescense Sensitivity: FITC 120 MESF, PE 110 MESF
Principal scientist: Mark Townley
The Knowledge Base contains forms, instruction and training material, minutes, policies, tools and other resources to support your research efforts by topic area.
- UIC: Online Instrument Reservation
- UIC: Flow Cytometer FAQs
- UIC: Training Form
- UIC: Sample Submission Form
- March 21, 2023: Flow Cytometer Workshop Materials
Contact Information
W123 Parsons Hall
Durham, NH 03824
Phone: (603) 862-2790
UNH Rate:
$59.00/hour assisted
$11.00/hour unassisted
Non UNH academic Rate:
$118.00/hour assisted
$22.00/hour unassisted
Industry Rate:
$150.00/hour assisted
$100.00/hour unassisted
