The NIKON A1R - HD Confocal microscope offers features for analyzing fluorescently labeled samples of various thickness and for creating 3D reconstructions.
The NIKON A1R - HD Confocal microscope is used for analyzing fluorescently-labeled or autofluorescing samples of various thicknesses, including automatically optically sectioning relatively thick specimens to create 3D reconstructions or 2D projections. Unlike conventional fluorescence microscopes, it produces images using only fluoresced light emitted at the focal plane (that is, in-focus fluorescence). Out-of-focus fluorescence emitted from above and below the focal plane is discarded. This microscope, therefore, can be used not only with relatively thin specimens, but with specimens that would typically be too thick (≥ 25 μm) to yield sharp images on conventional fluorescence microscopes.
Manufacturer: NIKON
Model #: A1R HD
Year of Manuf: 2017
Year Acquired: 2018
Location: Parsons Room W118A
The confocal microscope used was purchased by the University of New Hampshire's (UNH's) Center for Integrated Biomedical and Bioengineering Research (CIBBR) through a grant from the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH) (Grant P20GM113131) and is managed by UNH's University Instrumentation Center (UIC).
Specifications:
- LUNV laser launch with 405nm, 445nm, 488nm, 514nm, 561nm, 633nm solid state diode lasers (all 20mW output at the fiber tip)
- Hybrid resonant and Galvano scan head for simultaneous photoactivation and image acquisition
- The Perfect Focus System 4 corrects for focus drift, facilitating long-term observations
- Ti2 Inverted Microscope with 25mm FOV
- Motorized encoded XY stage, Piezo Z-axis drive
- Integrated and user-friendly NIS-Elements software for data acquisition and analysis
- 10x, 20x, 40x-oil and 60x-oil objectives
- Five detectors: two GaAsP and two multi-alkali PMTs, and spectral detector
- Color CMOS camera allows color non-confocal images to be collected (e.g. histological slides). Extended-depth-focus (EDF) software allows for in-focus color imaging of thicker samples.
- Stage inserts available to accommodate multiwell plates and four standard slides at a time
Principal Scientist: Mark Townley
The Knowledge Base contains forms, instruction and training material, minutes, policies, tools and other resources to support your research efforts by topic area.
- UIC: Online Instrument Reservation
- UIC: Confocal Microscope FAQs
- UIC: Confocal Microscope Brochure
- UIC: Sample Submission Form
- UIC: Cite CIBBR Support
W123 Parsons Hall
Durham, NH 03824
Phone: (603) 767-3267
UNH Rate:
$60.00/hour assisted
$13.00/hour unassisted
Non UNH academic Rate:
$120.00/hour assisted
$26.00/hour unassisted
Industry Rate:
$150.00/hour assisted
$100.00/hour unassisted
